Antibacterial Potential and Physicochemical Properties of Selected Yemeni Honeys Against Clinical Wounds Bacteria and Comparative with Standard Bacteria Isolates

Salwa H. Alkhyat¹ and Maher Ali Al.Maqtari²

¹Sana'a University, Faculty of Science, Department of Biology

²Sana'a University, Faculty of Science, Department of Chemistry

*Corresponding Author's Email: proteinase_613@hotmail.com

Accepted 19 April, 2014

Abstract

This study, the authors measured antibacterial activity and physicochemical properties of three available varieties of honey from Yemen, with six clinical wounds bacteria and their standard (Escherichia coli ATCC 10536, Staphylococcus aureus ATCC 29737, and Pseudomonas aeruginosa ATCC 25619) were tested for their susceptibility against Yemeni honey samples. The agar well diffusion assay demonstrated that, all honeys inhibit the growth of bacteria with clinical and standard of S. aureus then E. coli, while P. aeruginosa gave moderate growth with effect on its pigment production. Moreover, in all experimental approaches employed in the antibacterial test, it was revealed that, S. aureus was the most vulnerable organisms to these honey brands. There were no standard Attributes for Yemeni honeys and the researches are rare which describe its physicochemical Attributes (sugar, pH, conductivity, moisture, Hydroxymethylfurfural and phenolic content). Generally , The findings was showed that the Attributes of the studied honeys agree with international standards and some previous studies, with microorganisms have developed resistance  to many antibiotics, and this study will help in preparation of  novel antibacterial drugs using natural products.

Keywords: Yemeni honey, Antibacterial activity, Pathogenic organisms. Physicochemical properties.