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Vol. 3(3) April 2014
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Lawal AK
Dike EN
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Global Advanced
Research Journal of Microbiology (GARJM) ISSN: 2315-5116
April 2014 Vol.
3(3), pp 041-048
Copyright © 2014 Global Advanced
Research Journals
Full Length Research Paper
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Production of Alkaline Protease by Bacillus
polymyxa using Agro-Industrial Waste by Solid
State Fermentation
Lawal,
A. K1., *Itoandon, E. E1.,
Fashola, F1., Ajayi, O1., Ojo,
E. E1., Adepoju, F. A1.,
Ugbana, A. I1, Elemo, G. N1.
and Dike, E. N1
Enzyme Technology Division, Biotechnology Division
Federal Institute of Industrial Research P.M.B.
21023, Ikeja Lagos
Corresponding Author’s Email:
kolakuns@yahoo.com;
Tel: +234-8023164214
Accepted 02 April, 2014
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Abstract |
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The present study describes the screening of two
agro-industrial byproducts for enhanced production
of alkaline protease by Bacillus polymyxa
using solid state fermentation technique. The
agro-industrial byproducts were tested which include
soybean and rice bran for protease production.
Comparative studies on citrate buffer, borate buffer
and water for production and extraction of the
enzyme were also investigated. Of these byproducts
tested, soybean had higher activity at various
parameters (pH, temperature, metals, substrate
concentration and enzyme concentration). The effect
of borate buffer on the crude protease activity was
highest at pH 12, 1002.71; 60oC, 1593.56;
Mn2+, 1575.07; 2ml substrate
concentration, 1508.17 and 2ml enzyme concentration,
1744.04 respectively compared to the effect of
citrate buffer and water used during the
investigation.
Keywords: Bacillus polymyxa, Soybean,
Rice bran, Alkaline Protease, Assay.
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