Novel monoclonal biomarker antibody fragmentation collagen type iv to detect acute myocardial infarction related perviromonas gingivalis infection

Muliartha, Mulyohadi Ali and Djanggan Sargowo

Medical Faculty Brawijaya University

*Corresponding Author’s Email: ketutmuliartha@ymail.com

Accepted 18 November, 2013

Abstract

The objective of this study was to detect Acute Myocardial Infarction (AMI) in blood samples by using biomarkers for monoclonal antibody Collagen Type IV related Perviromonas Gingivalis. Rupture of atherosclerotic plaque initially occurs through the degradation of Collagen Type IV and infection is one important risk factor in AMI. This research was experimental, in vitro with a descriptive cross sectional design. The subjects were (n=4) healthy people and (n=12) AMI patients with ethical clearance and was carried out in the Biomedical Laboratory, Brawijaya University. To show the expression of MMP-9 and to show the fragmentation of Collagen Type IV. Western Blot was employed. The monoclonal antibodies were obtained from mice which had sub coetaneous immunization, followed by isolating lymphocytes and then fusing them with myeloma cells to form hybridoma. To evaluate the MMP-9 product and fragmentation of Collagen Type IV, Dot Blot technique was employed. The result found the following the MMP-9 products: the band indicated 92kDa and the Collagen Type IV fragmentation band 60-80 kDa. The test for biomarker reaction to biomarker monoclonal antibody fragmentation of Collagen Type IV in blood samples 1 to 12 was positive for AMI. In conclusion, the biomarker for Collagen Type IV monoclonal antibody fragmentation were AMI positive.

Keywords: Monoclonal antibody collagen type IV, AMI, biomarkers.