The first report of PVY incidence in Iran pepper fields

S.Mostafae1*, G.H.Mosahebi2  & M. Kuhi Habibi3

Collage of Horticultural Science & Plant Protection

University of Tehran,  Karaj. Iran.

Corresponding Authors E-mail: saghar_200059@yahoo.com 

Accepted 03 Mach, 2012

Abstract

Potato Virus Y(PVY) , Cucumber Mosaic Virus (CMV) and Alfalfa Mosaic Virus (AMV) are known as the main causes of sever systemic mosaic ,vein clearing ,vein necrosis and yellowing of 359 pepper samples were collected from pepper fields in Tehran province , using Double Antibody Sandwich Elisa (DAS-ELISA) and virus incidence for presence of CMV,PVY and AMV estimated . Biological properties including host range of the isolates was determined after biological purification. As this is the first report of PVY incidence in Iran pepper fields so we decided  to investigate characterization of PVY isolated from pepper. The result of ELISA, Immuno electrophoresis and Immuno Capture ReverseTranscription Polymerase Chain Reaction (RT-PCR) indicated that  pepper isolate of PVY shares the reported properties of the PVY in host range studies some differences were observed. for this reason the sequence analys and study of biological and molecular characterization of pepper-PVY isolated from Tehran pepper fields was performed.

Keywords: Potato Virus Y, Cucumber Mosaic  Virus, Alfalfa Mosaic Virus, Immuno Capture reverse transcription polymerase chain reaction (IC-RT-PCR), Immuno electrophoresis