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Antibacterial
efficacy of crude and diluted honey on four wound
isolates
W. Braide 1*,
S.U.Oranusi1., C.K. Akaluka 1,
R.N.Nwaoguikpe2., C.I.
Akobundu1 and N.I. Peter-Ikechukwu3
1Department
of Microbiology, Federal University of Technology,
PMB 1526, Owerri, Imo State, Nigeria.
2Department
of Biochemistry, Federal University of Technology,
PMB 1526, Owerri, Imo State, Nigeria.
3Department
of Food Science and Technology, Federal University
of Technology, PMB 1526, Owerri, Imo State, Nigeria.
*corresponding Author E-mail: wesleybraide2005@yahoo.com;
Phone +234-0803-7100-964
Received 27 January, 2012; Accepted 05 February,
2012
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The antimicrobials activities of crude and diluted
honey were determined against four clinical isolates
from surface and deep wounds. Staphylococcus
aureus, Pseudomonas aeruginosa, Klebsiella
pneumoniae and Escherichia coli were
isolated from wounds by routine microbiological
methods. Kirby- Bauer’s disc diffusion method was
employed in determining the susceptibility of
bacterial isolates to different concentrations of
honey. The results from the zone of inhibition
obtained (in mm) showed that the growth of all
isolates was completely inhibited by 20-100% honey
concentrations. The minimum inhibitory
concentrations (MIC) of honey for P. aeruginosa
and S. aureus were at 10 % (v/v) while that
of E.coli and K. pneumoniae were 20%
(v/v). The degree of susceptibility of the wound
isolates to honey was compared with that of ten
commercially available antibiotic discs. The result
obtained revealed that the susceptibility pattern of
honey at 40% (v/v) compared favourably and better
than amoxicillin, streptomycin, ceftriazone and
erythromycin.
Keywords:
Honey, antibacterial activities, wound bacteria
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