In vitro, controlling the establishment of Xanthomonas campestris with different bacterial bio-agents

Amna Ali*¹, M. Saleem Haider², Sobia Mushtaq¹, Ibatsam Khokhar¹, Irum Mukhtar¹, Sana Hanif² and Nosheen Akhtar²

¹First Fungal Culture Bank of Pakistan (FCBP), Institute of Agriculture Sciences (IAGS), University of the Punjab, Lahore, 54590, Pakistan.

²Institute of Agriculture Sciences (IAGS), University of the Punjab, Lahore, 54590, Pakistan

Corresponding author E-mail: write2amna@gmail.com

Accepted 18 July, 2012

Abstract

The antimicrobial agents of bacteria isolated from different rhizosphere of fruits and vegetables soil in Lahore. Often species, five were gram-negative (Escherichia coli, Pseudomonas fluorescence, Klebsiella pneumoniae, Salmonella typhii, Brachybacterium faecium); other five were gram positive and identified as Bacillus farraginis, Kurthia gibsonii, Aureobacterium liquefaciens, Curtobacterium albidum, Micrococcus lylae. The antagonistic potential of bacterial strains was assessed by the well diffusion technique and results indicating varying degree of biocontrol activity against pathogenic strain of X. campestris. Out of ten bacterial species, E. coli (gram negative) and C. albidum (gram positive) showed a high prevalence of resistance with reduction of 4.2cm and 4.1cm zone diameter respectively. The minimum inhibitory volume (MIV) to two bio-agents was determined for X. campestris from range 10-100 µL. E. coli (volume required to inhibit < 20 µL) and C. albidum (volume required to inhibit < 40 µL) exhibited good activity against pathogen. These results provide information on the prevalence of resistant bacterial strains with the MIV of organisms and indicate the possibility of using these bacterial species as bio-agent against X. campestris.

Keywords: Xanthomonas campestris, gram positive bacteria, gram negative bacteria