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Plasmid- mediated
aminoglycoside resistance of clinical isolates
of pseudomonas aeruginosa.
1*Oluwole Adebayo Daini and 2Chinyere
Gertrude Charles- Onyeaghala
1*Department
of Biochemistry, Obafemi Awolowo College of
Health Sciences, Olabisi Onabanjo University,
Remo Campus, P. M.B. 2005, Ikenne, Ogun State,
Nigeria.
2Department
of Medical Microbiology and Parasitology, Obafemi
Awolowo College of Health Sciences, Olabisi
Onabanjo University, Remo Campus, P. M. B 2005,
Ikenne, Ogun State, Nigeria.
1*Present
Address Dept. of Biochemistry, Kampala
International University, Western Campus, P.O.Box
71, Bushenyi, Uganda.
*Corresponding
Author Email:
ibioadaini@yahoo.com;
Tel: +256778032917
Accepted 03 May, 2012
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Out of one-hundred and sixty clinical specimens
obtained from different body sites at the University
College Hospital, Ibadan, after proper screening,
seventy yielded clinical isolates of Pseudomonas
aeruginosa. These were then subjected to
Antimicrobial Susceptibility testing and Plasmid
profiling. Twenty-seven clinical isolates [64.3%]
were found to be resistant to the Aminoglycoside
antibiotics tested; with Streptomycin being the
highest (30%) and Amikacin (12.9%) the lowest. A
total of twenty-two resistant strains harboured
plasmids ranging in Molecular sizes from o.55kb to
19kb and were grouped into five plasmid profiles.
Transformation experiment revealed that 407% of the
resistant strains carried a common R plasmid of size
19kb.Plasmid-Mediated resistance to Streptomycin,
Gentamycin and Amikacin were identified. We
therefore advocate a strict management of Antibiotic
policies in all Nigerian Hospitals.
Keywords: Amino glycosides, R-plasmids,
Pseudomonas aeruginosa.
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