Production and Optimal Performance Studies of Glucose Isomerase from Agricultural Raw Materials

*Lawal AK, Banjoko AM, Osikoya AF, Olatope SO, Kayode OF, Etoamaihe M, Emoleila I, Alebiosu

FA, Majolagbe YL , Shittu KA, Buhari F, and Dike EN.

Department of Biotechnology, Enzymology and Genetics Division. Federal Institute of Industrial Research Oshodi Lagos State Nigeria.

*Corresponding author E-mail: kolakuns@yahoo.com;  Tel.

+2348023164214

Accepted 06 August, 2012

Abstract

An Investigation was carried out to study the production and Optimal activity of glucose Isomerase enzyme from agricultural raw materials. A total of 81 Bacilli Isolates were isolated and thereafter screened for their ability to produce glucose isomearase by using the plate assay technique on Nutrient agar fortified with 4% (w/v) of glucose. Bacilli promising Isolates for Isomerase production were identified as Bacillus coagulans (B₁8, C₂1, C₂6, B₂13, C₃5 and C₂4) and Bacillus megaterium (A₃1,C₃9, B₃1, B₁1 and B₁7). Studies on the optimal conditions for the performance for temperature and pH of the produced Isomerase enzyme was in a range of 30⁰C - 70⁰C and 5.0 - 9.0 respectively. Comparative Studies of the enzyme for its activity as produced by Isolate B₁8 and C₃9 was 0.488µ/ml and 0.762µ/ml to 0.647µ/ml and 0.732µ/ml respectively. These values compares favourably with the standard (Sweetzyme) with 0.893µ/ml.

Keywords: Bacilli, agricultural raw materials , glucose isomerase, optimal condition.