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Isolation and typing of Clostridium spp.16S
rRNA from soil samples obtained in areas with sudden
mortality history in Colombia
Diego Ortiz Ortega, Luis Carlos Villamil Jiménez,
Rodrigo Martínez S.
Universidad de La Salle.
Cra. 7 172.85.
*Corresponding Author E-mail:
coramartinez@corpoica.org.co;
dortizo@unal.edu.co;
dortiz@corpoica.org; Tel 57-4227300
Accepted 09 April, 2012
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A Longitudinal epidemiologic
study was developed for the isolation, biochemical
characterization and molecular typing the bacterial
pathogen Clostridium spp found in soil from
areas affected by bovine sudden mortality.
Weincluded 10 herds, located in three
localities. The genetic variability of
Clostridium genus was analyzed by DNA sequence
of a 1500 bp fragment from the 16S rRNA gene.
Twenty four Clostridium isolates were biochemically
classified as Clostridium sordellii (41,7%),
Clostridium glycolicum (12,5%), Clostridium
hastiforme (12,5%); Clostridium botulinum
(8,3%), Clostridium butyricum (8,3%);
Clostridium chauvoei (4,3%),Clostridium
limosum (4,3%), Clostridium septicum (4,3%) and
Clostridium tertium (4,3%). The bacteria that
showed pathogenic activity were studied further by
16S rRNA gene sequencing at, which 55.5% was
classified as C. botulinum, where the native
strain isolated from areas with outbreaks was found
in a different group from Clostridium spp.
used by commercial laboratories for vaccine
production. Additionally, the native strain
identified here differs from others reported in Gen
Bank, indicating that the native pathogenic
Clostridium spp. is genetically different
to other Clostridium spp. used to
prepare immunogens affecting vaccine
efficiency. Our results indicate that the use of
native strains could improve commercial vaccine
preparations, increasing bovine immune response.
Key words: Clostridium, 16S rRNA ,
sudden mortality, genetic variability
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