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February 2015 Vol. 4 Issue
2
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Manjarrez-Gutierrez G
Hernandez-Rodriguez J
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Global Advanced Research Journal
of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
February 2015 Vol. 4(2), pp.
083-091
Copyright © 2015 Global Advanced
Research Journals
Full Length Research Paper
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Characterization of an intrinsic serotonergic system
in rat heart
Gabriel Manjarrez-Gutiérrez1,2*, Teresa
Neri-Gómez1, Alfonso Boyzo-Montes de Oca3
and Jorge Hernández-Rodríguez3
1Laboratory
of Molecular Pathology, Cardiology Hospital,
National Medical Center (CMN-SXXI),
Mexican Institute of Social
Security (IMSS), Mexico City, Mexico
2Medical
Research Unit in Neurological Diseases, Specialties
Hospital, CMN-SXXI, IMSS, México, DF, México
3Laboratory
of Neurontogeny, Department of Physiology,
Biophysics and Neurosciences, Center of Research and
Advanced Studies (CINVESTAV) IPN, Mexico City,
Mexico.
*Corresponding Author E-mail:
willisga@prodigy.net.mx;
gmanjarrezg@gmail.com; Phone: (+52) (55)
56276900, Ext. 22156; Fax:
(+52) (55) 55780240
Accepted 10 February, 2015
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Abstract |
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The objective of this study was to characterize
biochemically and immunohistochemically an intrinsic
serotonergic system in rat heart. Male Wistar rats
were used with an average body weight of 250 ± 10 g.
Rats were adapted for 2 weeks to standard animal
laboratory conditions. Hearts were later obtained to
determine the principal components of the
serotoninergic system using immunohistochemistry and
Western blot for tryptophan-5-hydroxylase (TPH) 1 or
2, serotonin transporter (SERT) and serotoninergic
receptors 5-HT1B, 5-HT2A, 5-HT2B
and 5-HT4. Activity of TPH, L-tryptophan
(L-Trp), 5-hydroxytryptamine (serotonin, 5-HT) and
5-hydroxyindolacetic acid (5-HIAA) were also
determined by high-resolution liquid
chromatography. Immunopositive cardiomyocytes for
both isoforms of TPH were observed. There was a
greater expression of TPH2in relation to TPH1.
Immunoreactivity for SERT and 5-HT1B,
5-HT2A, 5-HT2B and 5-HT4receptors
was also observed in cardiomyocytes. TPH activity,
L-Trp, 5-HT and 5-HIAA concentrations were also
demonstrated in heart tissue.These findings support
the fact that cardiomyocytes synthesize, utilize and
reuptake 5-HT, which constitutes an intrinsic
serotoninergic system that actively participates as
an autocrine and paracrine modulation system of
heart activities.
Keywords:
Cardiomyocytes, serotonin, tryptophan-5-hydroxylase,
SERT, serotoninergic receptors
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