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March
2013 Volume 2 Issue
3
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Sayed AEH
Mekkawy IA
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Gobal Advanced Research Journal
of Environmental Science and Toxicology (GARJEST) ISSN:
2315-5140
March 2013 Vol. 2(3), pp.
107-118
Copyright © 2013 Global Advanced Research Journals
Full Length
Research Paper
Genotoxicity induced by
4-Nonylphenol in adult and embryos of Clarias gariepinus
Alaa El-Din H. Sayed1,
Usama M. Mahmoud1
and Imam A. Mekkawy2
1Zoology
department, Faculty of Science, Assiut University,
71516Assiut, Egypt.
2Biology
department, Faculty of Science, Taif University, Taif, Saudi
Arabia.
*Corresponding Author E-mail:
alaa_h254@yahoo.com; Tel: +020882412381; Fax:
+020882342708
Accepted 08 December, 2012
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Abstract |
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The presence of the genetic toxic
chemicals in the aquatic environment increased. This
study aimed using the random amplified polymorphic
DNA (RAPD-PCR) assay to investigate the genotoxic
effects of 4-nonylphenol in adults and embryos of
African catfish
Clarias gariepinus.
After exposure of adults and embryos to sublethel
concentrations of 4-nonylphenol (0, 0.05, 0.08, and
0.1 mg/l) for 15 days, DNA was extracted for RAPD
assay. The RAPD pattern from catfish exposed to
4-nonylphenol displayed some changes in polymorphism
band patterns including disappearance and appearance
of bands. Cluster method was used to indicate the
distinct distance between the band patterns of
exposed fish and the control.
In conclusion, the RAPD-PCR is method can be used as
an investigation tools for the evaluation of the
genetic damage of the catfish induced by the
exposure to 4-nonylphenol.
Keywords:
4-nonylphenol, genotoxicity, embryos, Clarias,
gariepinus, DNA.
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